other:Article Title: AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.
Article Snippet: The following primary antibodies were used: anti-AIDA (Abcam, cat. no. ab199323; 1:500), anti-AIDA (Proteintech, 23724-1-AP; 1:200), anti-p-AIDA S161 (self-generated; 1:500), anti-HA (Proteintech, cat. no. 51064-2-AP; 1:3,000), anti-HA (F-7) (Santa Cruz Biotechnology, cat. no. sc7392; 1:150), anti-c-Myc (9E10) (Santa Cruz Biotechnology, cat. no. sc-40; 1:500), anti-PKA c-α (Cell Signaling Technology, cat. no. 4782; 1:1,000), anti-DYKDDDDK tag (Cell Signaling Technology, cat. no. 14793; 1:1,000), anti-β-tubulin (Cell Signaling Technology, cat. no. 2128; 1:2,000), anti-β3-tubulin (Cell Signaling Technology, cat. no. 5568; 1:1,000), anti-TH (Cell Signaling Technology, cat. no. 2792; 1:1,000), anti-p-TH S40 (Cell Signaling Technology, cat. no. 2791; 1:1,000), anti-β-actin (Cell Signaling Technology, cat. no. 3700; 1:5,000), anti-SDHA (Cell Signaling Technology, cat. no. 11998; 1:10,000), anti-GPAT3 (Proteintech, cat. no. 20603- 1-AP; 1:1,000), anti-MOGAT2 (Proteintech, cat. no. 19514-1-AP; 1:1,000), anti-DGAT2 (C-15) (Santa Cruz Biotechnology, cat. no. sc-32400; 1:1,000), anti-CALNEXIN (Proteintech, cat. no. 10427-2-AP; 1:1,000), anti-SIGMAR1 (Santa Cruz Biotechnology, cat. no. sc-137075; 1:1,000), anti-HSP90 (C45G5) (Cell Signaling Technology, cat. no. 4877; 1:2,000), anti-NRF2 (Proteintech, cat. no. 16396-1-AP; 1:1,000), anti-GCLC (Proteintech, cat. no. 12601-1-AP; 1:1,000), anti-TOM20 (Proteintech, cat. no. 11802-1-AP; 1:2,000), anti-TIM23 (Proteintech, cat. no. 11123-1-AP; 1:2,000), anti-NDUFV2 (Proteintech, cat. no. 15301-1-AP; 1:10,000), anti-UQCRC2 (Proteintech, cat. no. 14742-1-AP; 1:10,000), anti-COX4 (Proteintech, cat. no. 11242-1-AP; 1:10,000), anti-ATP5A (Proteintech, cat. no. 66037-1-Ig; 1:10,000), anti-VDAC1/2 (Proteintech, cat. no. 10866-1-AP; 1:3,000), anti-ATGL (Cell Signaling Technology, cat. no. 2138; 1:1,000), anti-p-HSL S660 (Cell Signaling Technology, cat. no. 4126; 1:1,000), anti-HSL (Cell Signaling Technology, cat. no. 4107; 1:1,000), anti- UCP1 (D9D6X) (Cell Signaling Technology, cat. no. 14670; 1:200), anti-UCP1 (Abcam, cat. no. ab10983; 1:3,000) and anti-UCP1 (C-17) (Santa Cruz Biotechnology, cat. no. sc-6528; 1:200).
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